The main objective of the present study is to predict characteristics of three dimensional pulstitile flow by location of stenosis in blood vessel with the second order bifurcation. The present study simulates the incompressible non-Newtonian laminar blood flows using a Fluent V. 6.0. The Carreau model is employed as the constitutive equation for blood. The numerical simulation carried out at five cases without and with symmetry or asymmetry stenosis. It is found that the no stenosis and stenosis before first bifurcation do not have influence on flow at second bifurcated blood vessel. However, the stenosis after first biburcation has effect on flow at second bifurcated blood vessel.