Due to emission of vehicles during loading/unloading, ventilation system in Roll-on/Roll-off ship is inevitable; however it is very difficult to predict the ventilation performance before it is finally built in. Although the requirements for the ventilation system include air change rate and maximum allowable concentration of CO in the cargo holds, even prototype tests are hardly able to quantify the ventilation performance. In the present paper, a new method to assess the ventilation performance of Roll-on/Roll-off ship is proposed by using computational fluid dynamics. The air exchange is modeled by introducing multi-species transport of existing air In the holds and new air from the ventilation system. Conservation of multi-species as well as 3D Navier-Stokes equation are solved numerically in time dependent manner. Several cases of different configuration are considered. The results include predicted mass fraction of new air in the holds. It is also presented that CO concentration can be estimated based on the predicted air change performance. Due to the lack of experimental data, the computed results are not verified; however the proposed method can be applied as au assessment tool