Chitosan pre-treatment of the fabrics prior to the dyeing processes has been reported to increase the uptake of natural dyestuffs. In this study, cotton, nylon, and PET fabric specimens were pre-treated with chitosan prior to the dyeing (Method 1), or the state of chitosan acid salt formation, coated on the yarn surface, was destroyed prior to the dyeing process by alkaline neutralization process (Method 2). In case of the acid salt formed cotton (Method 1), treated fabrics showed more yellowish color component of cochineal, while alkali-treated (Method 2) cotton showed more uptake of bluish color of cochineal.