In order to investigate the effects of bisphenol A (BPA) on cell mediated immune reaction in vitro we examined the allogenic mixed lymphocyte reaction (MLR), splenocytes proliferation (SP) to T cell mitogens and IFN-${gamma};production$. Splenocytes of Balb/c mice ($1.5{ imes}10^5$ cells/well) were co-cultured with different numbers of mitomycin C-treated mature dentritic cells (DCs) in presence of BPA (25, 50, 100 ${mu}M$) and $[^{3}H]$thymidine incorporation (cpm) was measured by scintilation counting. Splenocytes ($2{ imes}10^6$ cells/well) were cultured with mitogens, Con A ($2;{mu}g/ml$), PHA ($5;{mu}g/ml$) and IL-2 ($0.1;{mu}g/ml$), or PMA ($5;{mu}g/ml$) and INO ($1;{mu}g/ml$) in presence of BPA (1, 10, 25, 50, 100 ${mu}M$) and SP was assessed by MTT assay. $IFN-{gamma}$ levels in culture supernant were determined by ELISA. At low concentration, BPA slightly increased MLR, SP and $IFN-{gamma}$ levels, but at higher concentration it showed significant inhibitory effects on these immunological parameters. These results indicate that BPA is able to alternate cell-mediated immune reaction.