Ethylene glycol (EG) intoxication in dogs can pose a diagnostic challenge. We investigated the effect of a lethal dose of EG to the Shepherd on blood, urine test, and histopathologic observation. Shepherds were treated orally with EG at $10;m{ell}/kg$ body weight. Clinically, EG caused the vomition, depression of central brain. Anion gap was increased, which was decreased pH of blood, The blood osmorality was increased at 3 hours, and BUN and creatinine was increased at 24 hour significantly. The pH of urine decreased. The crystal of calcium oxalate appeared at 6 hours. The amount of crystals increased at 12 and 24 hours. Microscopically, the degeneration of proximal tubules were shown at 1 hour and finally progressed as acute tubular nephrosis at 24 hours. These results suggest that the blood and urine test accompanied with histopathological examination be helpful to investigate the EG intoxication.