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Genotypic Characterization of Salmonella enterica Serotype Enteritidis Isolated from Food-Poisoning Cases and Chickens by Pulsed Field Gel Electrophoresis
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  • Genotypic Characterization of Salmonella enterica Serotype Enteritidis Isolated from Food-Poisoning Cases and Chickens by Pulsed Field Gel Electrophoresis
  • Genotypic Characterization of Salmonella enterica Serotype Enteritidis Isolated from Food-Poisoning Cases and Chickens by Pulsed Field Gel Electrophoresis
저자명
Suh. Dong Kyun,Song. Jae Chan
간행물명
Journal of experimental & biomedical sciences
권/호정보
2005년|11권 1호|pp.9-13 (5 pages)
발행정보
대한의생명과학회
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정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

A total of 22 Salmonella enterica serotype Enteritidis (S. Enteritidis) isolates from human and chicken sources were analyzed by pulsed field gel electrophoresis (PFGE) using XbaI restriction enzyme to assess the genetic relationships between strains from different sources. PFGE permitted the resolution of XbaI restriction fragments of the 22 S. Enteritidis into 6 distinct PFGE types (PFT), designated PFT1 to PFT6, and 2 subtypes within PFT2, and allowed to detect between 9 and 10 bands with fragments sizes in the range of $25~635;kb$. Four of twelve isolates from human showed an identical PFGE patterns with 2 isolates from chickens. Also, another one isolate from human showed an identical PFGE patterns with other 5 isolates from chickens. Only one isolate from chicken, however, showed a different pattern compared to other PFTs. These results suggested that sporadic human food-poisoning cases infections caused by S. Enteritidis in this study were due to the consumption of contaminated chicken meats and that a clonally highly similar strains exist and spread between human and chicken sources.