Crack tip displacement is originated by tensile stress component, s and shear stress component, t on pure Mode I and pure Mode II. The crack tip displacement(CTD) depends on combined types of different two stress components under mixed-mode loading conditions (MMLC). Thus, the analysis of crack tip displacement must be CTD vector, dv which is composition of ds and dt under MMLC. In this paper, various effects of MMLC on the crack closure are studied experimentally. The crack closure magnitude is calculated from the information of crack tip displacement under MMLC. This information has been obtained from the high resolution optical microscope in direct observations of crack displacement behavior at the crack tip. Observed crack tip displacement is analyzed by using CTD vector to determine crack opening load. The various effects of MMLC on the crack closure are explained using crack opening ratio with crack length and mode mixture. The effective stress intensity factor considering crack closure is also discussed.