The goal of this research was to determine whether gender differences exist in impact force and impact shock variables at stance phase during a preferred running. Ten male and ten female subjects volunteered to participate in this study. Impact force was quantified by using a surface-mounted force plate. In addition, Axial accelerations of the tibias and mouth were measured using low-mass accelerometers. Comparison of parameters relating to impact force and impact shock which attained from time domain, and impact shock parameters which were analyzed in frequency domain were made between genders. The conclusions based on results were as follows; 1. There were no significantly differences in impact force, mouth and tibia acceleration peak in time domain between two genders. 2. The male group was greater in impact shock peak of PSD(power spectral density) at the tibia than female group(p<.05), but no differences in active impact of PSD at the tibia and the mouth between two genders. 3. Female subjects exhibited that a peak of impact shock attenuation analyzed in frequency domain moved toward a high frequency, but no difference in time domain between two genders.