Amynthas hupeiensis with 6/7/8/9 intersegmental spermathecal pores keys to the sieboldi-group in Sims and Easton (1972). Two pairs of round genital papillae are always between segments 17/18 and 18/19, and male pore regions are positioned in between the genital papillae. Korean Amynthas hupeiensis is usually collected from various agroecosystems. Description of the Amynthas hupeiensis is provided, including illustrations of ventral view and spermathecae, DNA barcoding data, and photo.