As the use of digital systems becomes more prevalent, adequate techniques for software specification and analysis have become increasingly important in nuclear power plant (NPP) safety-critical systems. Additionally, the importance of software verification and validation (V&V) based on adequate specification has received greater emphasis in view of improving software quality. For thorough V&V of safety-critical systems, V&V should be performed throughout the software lifecycle. However, systematic V&V is difficult as it involves many manual-oriented tasks. Tool support is needed in order to more conveniently perform software V&V. In response, we developed four kinds of computer aided software engineering (CASE) tools to support system specification for a formal-based analysis according to the software lifecycle. In this work, we achieved optimized integration of each tool. The toolset, NuSEE, is an integrated environment for software specification and V&V for PLC based safety-critical systems. In accordance with the software lifecycle, NuSEE consists of NuSISRT for the concept phase, NuSRS for the requirements phase, NuSDS for the design phase and NuSCM for configuration management. It is believed that after further development our integrated environment will be a unique and promising software specification and analysis toolset that will support the entire software lifecycle for the development of PLC based NPP safety-critical systems.