Objectives: This study was undertaken to determine if Plantaginis Semen(PS) has a protective effect against $CCI_4$ induced hepatoxicity in Rats. Methods: The experimental groups were divided into Normal group (untreated Group), Control group $(group;treated;with;CCI_4)$, Sample 1 (group treated with 0.1 ml Plantaginis Semen herbal acupuncture in both sides of $Gar{a}nshar{u};(BL_{18})$ after $CCI_4$ treating) and Sample 2 (group treated with feeding of 1 cc Plantaginis Semen extraction after $CCI_4$ treating). Rats were administered orally $CCI_4;(0.1;ml/kg)$ for 4 days. In experiments for PS effect, rats of Sample 1 group received 0.lml herbal acupuncture of PS extraction in both sides of corresponding $Gar{a}nshar{u};(BL_{18})$ of human body for 3 days after treated $CCI_4$. Rats of Sample 2 group feeded PS extraction (1cc) for 3 days after treated $CCI_4$. Variation of weight and biochemical assays (AST, ALT, LDH, ALP, total cholesterol, triglyceride, total protein, albumin) were performed. Results: In Control group, $CCI_4$ increased serum AST, ALT, LDH, ALP, total protein, albumin and decreased weight, total cholesterol, triglyceride. Sample 1 and Sample 2 significantly decreased serum AST, ALT, LDH, ALP, total protein, albumin as compared with Control group. Conclusion: These results indicate that PS could be used in prevention and treatment of hepatoxicity. However, precise mechanisms of PS protection remain to be determined.