Iron is the required microelement supporting life and is the main component of hemoglobin. Thus iron has affinity with exercise capacity. Iron metabolism turbulence induced by exercise is one of causes of hematopoietic hypofunction. Results of the experiment showed that long-term treadmill exercise of progressive loading significantly decreased levels of erythrocyte indexes, serum iron, serum ferritin and significantly increased serum transferrin level. Nutrition supplement could significantly retard the variations, and Exercise +Nutrition group have higher levels of erythrocyte indexes, serum iron, serum ferritin and lower level of serum transferrin than Exercise group. The results indicated that nutrition supplement have function of prevent and cure on iron metabolism turbulence induced by exercise, furthermore significantly enhance hemoglobin level in rats.