Takrisodokyeum (TRSDY) has been known to exert anti-tumoral activity in Korea. However, its molecular mechanism of action is not understood. In this study, we found that TRSDY induced apoptosis in androgen-independent prostate cancer PC-3 cells as evidenced by DNA fragmentation. Our data demonstrated that TRSDY-induced apoptotic cell death was accompanied by decreases of PAKT and $NF-{kappa}$ activation, which is resulted from inhibition of $I{kappa}B-{alpha}$ degradation. But TRSDY-induced apoptotic effect of PC-3 cells was independent of Par-4 expression. Taken together, these results suggest that TRSDY inhibits AKT phosphorylation and $NF-{kappa}B$ activation, and eventually leads to apoptotic cell death in androgen independent prostate cancer PC-3 cells.