Objects : This study has been carried out to assess the effects of the variable herbs on $IFN-{gamma}$ secretion in the mouse spleen cell. Methods : 202 kinds of herb extracts were used to evaluate the $IFN-{gamma}$ secretory distinction by each $1{mu}g/ml$ and $10{mu}g/ml$ density of water. All experimental herbs were grouped by oriental herbalogical method. But each herb had its independent variables. Results : The secretions increased in 20% of all herbal water. The density differences also make different effects on the secretion of $IFN-{gamma}$. The secretion of IFN-${gamma}$ inclosed in some kinds of herbs of IFN-${gamma}$. It has representatively increased in Imperaetae Rhizoma(白茅根) of $1{mu}g/ml$ and Notopterygii Rhizoma(羌活)of $10{mu}g/ml$. $IFN-{gamma}$ incresed in 12 kinds of heybs of both densities. The secretion of $IFN-{gamma}$ decreased in some kinds of herbs of $IFN-{gamma}$. It has representatively decreased in Moutan Radicis Cortex(蘇丹皮) of $1{mu}g/ml$ and Angelicae Radix(富歸尾) of $10{mu}g/ml$. $IFN-{gamma}$ decreased in 18 kinds of herbs of both densities. In t oriental herb group, The secretion of $IFN-{gamma}$ increased in Bang-Hyang-Hwa-Sup group(芳香化濕藥), He-Pyo group(解表藥), I-Su-Sam-SuP group(利水渗) The secretion of $IFN-{gamma}$ decreased in Gu-Chung group(驅蟲藥), An-Sin group(安神藥). Su-Sap group(收澁) On-Li group(溫裏藥), I-Gi group(利氣藥). Conclusions : The result of this study will not only broaden applications of oriental medicine to biological therapy, but also form the basis of oriental medical therapy to find out the meaning of oriental classification.