The effect(s) of the volatile oil (VO) of Nigella sativa and its two components, ${alpha}-pinene$ and ${ ho}-cymene$ on body temperature of male and female conscious mice were studied. Further investigations to delineate the mechanism(s) of action of the observed effect(s) by using various blockers involved in the central regulation of body temperature were made. VO and ${alpha}-pinene$ caused significant reductions in rectal body temperature at is and 30 minute after treatment. ${ ho}-cymene$ had negligible effect on body temperature of mice. Cyproheptadine inhibited VO and ${alpha}-pinene-induced$ hypothermia significantly. Nalbuphine inhibited ${alpha}-pinene-induced$ hypothermia significantly but did not affect VO-induced hypothermia. Droperidol potentiated VO and ${alpha}-pinene-induced$ hypothermia to a non-significant level; whereas atropine potentiated VO-induced hypothermia non-significantly. The study confirms further the role of serotoninergic receptors in the mechanism(s) of the observed pharmacological effects of the VO of Nigella sativa. It also indicated a possible role of opioid receptors in ${alpha}-pinene-induced$ hypothermia.