We have investigated electro-optical characteristics in three kinds of twisted nematic (TN) cells on the polyimide surface. The threshold voltage and the response time of thermal stressed TN cells were same that of no thermal stressed TN cells. There were little change of value in these TN cells. On the other hand, transmittance of no thermal stressed TN cells were better than that of thermal stressed TN cells. Transmittances of TN cells on the polyimide surface decreased by increasing thermal stress time. Moreover, the residual DC of the thermal stressed TN cells increased as increasing thermal stress temperature and time. Therefore, thermal stability of TN cells were decreased gradually by giving high thermal stress for a long time.