It is widely known that Environmental Tobacco Smoke(ETS) is not good for health. ETS is composed of a lot of chemicals. So indicators are needed to evaluate the risk of ETS in air. One of the indicators is Nicotine. Active sampler has been used to measure nicotine concentration in air. The experiments were conducted to compare the active sampler method with diffusive sampler in exposure chamber and smoking areas, respectively. Sampling rate was 40.5 ml/min in exposure chamber. Experimental sampling rate (40.5 ml/min) was more than theoretical sampling rate (33.52 ml/min). And the higher was the concentration in air, the higher was experimental sampling rate. The average desorption, rate was 113.6%. The overall precision was 7.31 %. The overall accuracy was 18.96%, which were under NIOSH criteria. The average(GM) concentrations of nicotine by two sampling methods were $8.29{mu}g/m^{3}$ (active sampler), $7.54{mu}/m^{3}$ (diffusive sampler) in smoking area and smoking room. There was no regression between active sampler and diffusive sampler ($R^{2}=0.2397$). But slope, coefficient of determination was 1.017, 0.9292, respectively after removing outliers. And the slope (1.017) was close to the theoretical slope (1). In conclusion, this study indicated that diffusive sampler can be used to evaluate concentration of nicotine in air instead of active sampler.