In order to estimate delivered nutrients load from non-point sources in the Gap stream watershed in Daejeon, a distributed watershed model SWAT2000 was used so that it could predict the impact of land management practices on water, sediments and chemicals yields in large complex watersheds with varing soils, land uses and management condition over long period of time. The SWAT2000 was applied to the Daejeon (Indong), Yudeung (Boksu) and Gap (Hoeduck) streams for TMDL (Total Maximum Daily Load) of nutrients. The observed water quality and streamflow data of the year of 2002 and 2003 were used for calibration, and those of the year 2004 and 2005 were used for validation. Simulated results were evaluated by Estimation Efficiency Analysis (COE), Regression Analysis $(R^2)$ and Relative Error (R.E.) for the nutrients amounts on the monthly and yearly basis by comparing observed load with estimated load obtained by using SWAT2000 simulations. The COE value fur T-N was ranged from 0.59 to 0.78, $R^2$ values for T-N ranged from 0.65 to 0.84, and R.E values fur T-N load ranged from 4% to 20%. COE value far T-N was ranged from 0.59 to 0.73, $R^2$ values for T-P ranged from 0.67 to 0.82, and R.E values for T-P load ranged from 3% to 25%. Estimated results of SWAT2000 simulation for 3 sites (Indong, Boksu, Hoedeok) were reasonably satisfactory. This study indicated that SWAT2000 model could be applicable to estimate the nutrients load from the Gap stream watershed in Korea.