The purpose of this experimental study is to investigate formant characteristics of Korean monophthongs spoken by Japanese learners and to compare the characteristics of vowels produced by the Japanese learners with those of the Korean native speakers. The data consisted of three categories: eight vowels in isolation, words including eight vowels in carrier sentences, and words including eight vowels in natural sentences. In this study, formant frequencies of the vowels were measured by Wave Surfer. It was assumed that the formant frequencies of the Korean vowels produced by the Japanese learners could be different from those of the Korean native speakers due to the influence of their own Japanese vowels. Results of this study showed that the Japanese learners had the difficulties to distinguish between the pairs /-/and /ㅜ/, /ㅓ/and /ㅗ/, and /ㅏ/and /ㅔ/. In Japanese vowels, F2 frequency value of /ㅜ/ was similar to that of the Korean /-/. It means that when the Japanese leaners produced Korean /ㅜ/, they might neutralize /-/ and /ㅜ/. Besides, there were not /ㅓ/and /ㅐ/ in Japanese vowels. Therefore, they tended to pronounce /ㅓ/ similar to /ㅗ/ which has the most similar formant frequency value with that of /ㅓ/, and /ㅐ/ was pronounced similar to /ㅔ/ for the same reason.