Wildlife roadkill is a serious problem for road planners and biologists concerned with traffic safety, species conservation, and animal welfare. The objective of this paper was to analyze the effect of land use on the frequency of mammal roadkills. This study took part in three steps. First, data for 1,279 road kills of nine species were collected by survey over 26 months on 107km of roads near the Mt. Jirisan area. Second, the type of land use near the roadkill points was analyzed, and the area of each land use within loom of the road was calculated using GIS. Finally, the question of whether the land use affects the Sequency of mammal roadkills was analyzed by determining the density of roadkill occurrences for each land-use type. The results of the study were as follows. First, the roadkill density of all species was highest near grasslands, especially natural grasslands. Second, when comparing forests and rice fields, roadkill densities of the Eurasian red squirrel, Siberian chipmunk and Korean hare were higher near the forests, while the densities of the raccoon dog, water deer and leopard cat were higher near rice fields. The results of this study could be an essential information source for mitigating the roadkill problem in Korea.