Heterotrimeric GTP binding proteins (G proteins) mediate signal transduction generated by neurotransmitter and hormones. Among G-proteins, Go is classified as a member of the Go/Gi family and the most abundant heterotrimeric G protein in brain. Most of the mechanistic analyses on the activation of Go indicated its action to be mediated by the $G{eta}{gamma}$ dimer because downstream effectors for its ${alpha}$ subunit have not been clearly defined. To determine the downstream effectors of alpha subunits of Go ($Go{alpha}$), we used yeast two-hybrid system to screen $Go{alpha}$ interacting partners in cDNA library from the human brain. A brain specific high mobility group box containing protein (BHX), A possible transcription factor, was identified as a $Go{alpha}$ interacting protein. We confirmed interaction between $Go{alpha}$ and BHX employing in vitro affinity binding assay. Moreover, active form of $Go{alpha}$ preferentially interacts with BHX than inactive farm. Our findings indicate that $Go{alpha}$ could modulate gene expression via interaction with BHX during neuronal or brain development.