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Cryopreservation of Somatic Embryos of Soapbeny (Sapindus mukorossi Gaertn.) by Vitrification
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  • Cryopreservation of Somatic Embryos of Soapbeny (Sapindus mukorossi Gaertn.) by Vitrification
  • Cryopreservation of Somatic Embryos of Soapbeny (Sapindus mukorossi Gaertn.) by Vitrification
저자명
Kim. Hyun-Tae,Yang. Byeong-Hoon,Park. Young-Goo
간행물명
韓國資源植物學會誌
권/호정보
2006년|19권 6호|pp.665-669 (5 pages)
발행정보
한국자원식물학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

Somatic embryos do not survive at exposure to liquid nitrogen temperatures without cryoprotective treatments. A simplified technique which simultaneously induces and cryoprotects embryogenic calli using plant vitrification solution 2 (PVS2) followed by dehydration was developed for the cryopreservation of Soap berry genetic resources. Vitrification is a way of removing the moisture in vegetation through PVS2. The PVS2 vitrification solution consisted of 30% glycerol (w/v), 15% ethylene glycol (w/v), 15% Dimethylsulfoxide (w/v) in B5 medium containing 0.4M sucrose. Two tests were done. The one was to eliminate moisture at $0^{circ}C$ and the other at $25^{circ}C$. In both cases the best results came out at a vitrification time of $10{sim}20$ minutes. It was also found that the survival rate was higher at $0^{circ}C$ than at $25^{circ}C$. In particular, the survival rate reached more than 80%. Water-damaged embryos turned brown and stoped growth, but energetic embryos took on a milky hue and show a very vigorous growth rate. Successful cryopreservation of somatic embryos of soapberry can be used to establish in vitro genebanks for long-term conservation of Soapberry genetic resources to complement field genebanks and other in vitro methods already being used.