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Kinetics and Modelling of Cell Growth and Substrate Uptake in Centella asiatica Cell Culture
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  • Kinetics and Modelling of Cell Growth and Substrate Uptake in Centella asiatica Cell Culture
  • Kinetics and Modelling of Cell Growth and Substrate Uptake in Centella asiatica Cell Culture
저자명
Omar. Rozita,Abdullah. M.A.,Hasan. M.A.,Rosfarizan. M.,Marziah. M.
간행물명
Biotechnology and bioprocess engineering
권/호정보
2006년|11권 3호|pp.223-229 (7 pages)
발행정보
한국생물공학회
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정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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In this study, we have conducted kinetics and modelling studies of Centella asiatica cell growth and substrate uptake, in an attempt to evaluate cell growth for a better understanding and control of the process. In our bioreactor cultivation experiment, we observed a growth rate of 0.18/day, a value only 20% higher than was seen in the shake flask cultivation trial. However, the observed maximum cell dry weight in the shake flask, 10.5g/L, was 14% higher than was achieved in the bioreactor. Ninety seven percentage confidence was achieved via the fitting of three unstructured growth models; the Monod, Logistic, and Gompertz equations, to the cell growth data. The Monod equation adequately described cell growth in both cultures. The specific growth rate, however, was not effectively predicted with the Logistic and Gompertz equations, which resulted in deviations of up to 73 and 393%, respectively. These deviations in the Logistic and Gompertz models may be attributable to the fact that these models were developed for substrate-independent growth and fungi growth, respectively.