The aim of this study is to investigate in vitro biological properties of Eucommia ulmoides Oliv. bark. Ethyl acetate(EtOAc) fraction from aqueous extract of Eucommia bark showed strong antioxidant activity of $IC_{50}$ 19.2 ${mu}g/ml$ by 1,1-diphenyl-2-picrylhydrazyl(DPPH) free radical-scavenging assay. The Eucommia bark extract showed $alpha$-glucosidase inhibitory activity and inhibited growth of human liver cancer cell, suggesting its potential biological value of anticancer.