High performance liquid chromatography (HPLC) was used for the analysis of the lignans eleutheroside B and eleutheroside E in the fruits of Acanthopanax species. A reverse-phase system using a gradient of $H_2O$ and acetonitrile as the mobile phase was developed and detection was at 210 nm. The analysis was successfully carried out within 20 min. The content of eleutheroside B and eleutheroside E in Acanthopanax species was measured in the fruits of A. senticosus (0.58 and $1.66;{mu}g/mg$, respectively), A. sessiliflorus (1.15 and $8.49;{mu}g/mg$, respectively), A. koreanum (2.16 and $1.80;{mu}g/mg$, respectively), and A. divaricatus (1.06 and $7.08;{mu}g/mg$, respectively).