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Transformation of Leuconostoc mesenteroides SY1, a Strain Isolated from Kimchi
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  • Transformation of Leuconostoc mesenteroides SY1, a Strain Isolated from Kimchi
  • Transformation of Leuconostoc mesenteroides SY1, a Strain Isolated from Kimchi
저자명
JEONG. SEON-JU,PARK. JAE-YONG,KIM. JONG HWAN,KIM. GYEONG MIN,CHUN. JIYEON,LEE. JONG-HOON,CHUNG. DAE-KYUN,KIM. JEONG HWAN
간행물명
Journal of microbiology and biotechnology
권/호정보
2006년|16권 1호|pp.149-152 (4 pages)
발행정보
한국미생물생명공학회
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정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

Leuconostoc mesenteroides SY1, a strain isolated from cabbage Kimchi, was transformed with pCW4, a shuttle vector based on a cryptic plasmid from Lactobacillus paraplantarum C7. $alpha-Amylase$ gene, amyL, from Bacillus licheniformis was cloned into pCW4, resulting in $pCW4T{alpha},;and;pCW4T{alpha}$ was introduced into SY1 by electroporation. Transformation efficiency was $10^2cells/{mu}g$ plasmid DNA. L. mesenteroides cells harboring $pCW4T{alpha}$ did not show amylase activity, although amyL transcript was synthesized as determined by slot blot experiment. $pCW4T{alpha}$ was stably maintained in SY1 in the presence of erythromycin (Em, $5;{mu}g/ml$) but rapidly lost when Em was omitted. Less than $1\%$ of the cells maintained $pCW4T{alpha}$ after 5 days at $30^{circ}C$.