Most projects of tideland reclamation with dike construction produce estuarine reservoirs, which may result in water quality problems due to blocking of natural flow of stream water to the sea. External loadings to the reservoirs through tributaries are major concerns in a concerned water quality management. The water quality of a reservoir is greatly influenced by watershed drainage, and accurate estimation of pollutant is indispensable for in the reservoir management. Concentrations of the microorganisms in stream water and conventional parameters were monitored in the 13 water quality monitoring sites located in a rural watershed of Hwaong estuarine reservoir. The indicator of microorganisms showed strong correlation between them, and regression equations with $R^2geq0.70$ may be used fur estimating one from other microorganisms. The relationships between water quality parameters obtained in this study may be used to infer one unknown pollutant concentrations from the measured pollutant loadings. This methodology could be applied to other areas where the watershed characteristics are not significantly different from the study area. High concentrations of nitrogen was observed in water quality monitoring sites affected by urban land uses and numbers of livestock in wet day as well as dry day, due to the influent of diffuse sources.