Purpose: This study was conducted to survey knowledge, depression, and quality of life of mothers with hemophilic children, and to provide basic data for a health promoting intervention improving their quality of life. Method: The subjects of this study were 140 mothers of hemophilic children by convenience sampling. The collected data were processed using SPSS program and analyzed using descriptive statistics, and Pearson correlation. Result: The knowledge of mothers with hemophilic children was 2.8 points out of 4 points. The depression of the subjects was 2.1 points out of 4 points. The quality of life of the subjects was 3.2 points out of 5 points. Results also showed a positive relationship between quality of life and knowledge (r=.45, p<.001) and a negatives relationship between quality of life and depression (r=-.41, p<.001). Conclusion: Knowledge and depression affect quality of life of mothers with hemophilic children. Therefore, it is necessary to develop a nursing intervention with these variables to increase the quality of life for mothers with hemophilic children.