The present study evaluated the accuracy of MSD-related work criterion of Korea Ministry of Labor by using ergonomic workload evaluation methods. 1,948 manufacturing works in automobile industry were divided into 1,286 MSD-related risky works and 662 risk-free ones by using RULA, ANSI Z-365 and perceived discomfort evaluation. Then, the two types of works were evaluated by Labor Ministry work criterion to analyze the classification accuracy of the work criterion. The work criterion of Korea Labor Ministry had low hit and false alarm ratio(20 and 11%) and high miss and correct rejection ratio(80 and 89%), and the odd ratios of hit and correct rejection were 0.2 and 8.3, respectively. It can be concluded that the work criterion of Korea Labor Ministry is a conservative evaluation method which tends to evaluate a target work as risk-free.