Separation motion analysis of staging system is conducted using combined analysis programs, which include unsteady aerodynamic analysis codes and dynamic motion analysis tools. In this study, the analysis is for the long-rang missile staging system. The purpose of this study is to verify the safety and reliance of the proposed staging system, and to find out the influence of angle of attack perturbation on staging. A structured parallel overset mesh called Chimera grid is used for the simulation of unsteady supersonic Euler flow solver. In addition, unsteady dynamic simulations are also performed.