유자씨앗 중의 플라보노이드 및 리모노이드 화합물을 methanol 및 ethanol로 추출 HPLC로 정량한 결과 ethanol추출의 경우가 거의 모든 화합물에서 높게 나타났고 화합물의 종류도 더 많이 검출되었다. Limonin이 methanol 추출의 경우 140.34 mg/100g, ethanol 추출의 경우 170.98 mg/100g으로 개별화합물 중 가장 높은 함량을 나타내었고 그다음이 caffeic acid, naringin, lutin, nomilin 등의 순으로 높았다. HPLC/MS에 의하여 약42가지 화합물의 분자량 측정이 가능하였으며 mass spectrum과 extracted current ion chromatogram으로 분자량 측정이 가능하였다.
Flavonoid and limonoid compounds were determined by HPLC on the methanol and ethanol extracts from citron seeds. The quantities of the compounds in these categories were higher in the ethanol extract than methanol extract. The types of these compounds were detected in larger numbers in the ethanol extract. The content of limonin was the largest in both methanol and ethanol extract among the detectable compounds ; 140.34 mg/100g and 170.98 mg/100g, respectively, and the contents of other compounds, caffeic acid, naringin, lutin, nomilin, were found in large amount in this order. The molecular weights of forty two compounds in ethanol extract were determined with mass spectrums and extracted ion current chromatograms by HPLC/MS.
