The aim of this study is to clarify the therapeutic effect of hemodialysis (HD) on acute renal failure in dogs experimentally induced by ethylene glycol (EG) ingestion in experiment 1(HD on 12 and 36 hours after EG injestion) and experiment 2(HD on 24 and 48 hours after EG injestion). Clinical signs such as vomiting, depression, ataxia and knuckling were improved on experiment 1 and 2. White blood cell (WBC) and platelets (PLT) were decreased, and packed cell volume (PCV) was increased after hemodialysis, however, no significant differences were observed in experiment 1 and 2. BUN, creatinine and phosphorus was significantly decreased in experiment 1 and 2, respectively. These results suggested that hemodialysis was effective for acute renal failure in dogs induced by EG ingestion.