The Holographic Particle Velocimetry system can be a promising optical tool for the measurements of three dimensional particle velocities. One of inherent limitations of particle holography is the very long depth of field of particle images, which causes considerable difficulty in the determination of particle positions in the optical axis. In this study, we introduced three auto-focusing parameters corresponding to the size of particles, namely, Correlation Coefficient, Sharpness Index, and Depth Intensity to determine the focal plane of a particle along the optical axis. To investigate the suitability of the above parameters, the plane image of dot array screens containing different size of dots was recorded by diffused illumination holography and the positions of each dot in the optical axis were evaluated. In addition, the effect of particle position from the holographic film was examined by changing the distance of the screen from the holographic film. All measurement results verified that the evaluated positions using suggested auto-focusing parameters remain within acceptable range of errors. These research results may provide fundamental information for the development of the holographic velocimetry system based on the automatic image processing.