In this paper, post-earthquake capacity evaluation method of reinforced concrete buildings was studied. Substructure pseudo-dynamic test and static loading test of first story column in a four-story R/C building was carried out in order to investigate the validity of the evaluation method proposed in the Damage Assessment Guideline (JBDPA 2001). In pseudo-dynamic test, different levels of damage were induced in the specimens by pre-loading, and input levels of seismic motion, at which the specimens reached to the ultimate stage, were examined. From the experimental result, no significant difference in damage levels such as residual crack width between the specimens under static and pseudo-dynamic loading was found. It is shown that the seismic capacity reduction factors ${eta}$ can provide a reasonable estimation of post-earthquake seismic capacity of R/C buildings suffered earthquakes.