Wear of engine valves and seat inserts is a major factor affecting engine performance. In order to improve quality and life of valve assemblies, wear mechanism and 3-D surface topography should be analyzed according to operating conditions of the engine. After developing an engine simulator that generates valve speed up to 90Hz and temperature up to $900^{circ}C$ as well as controls test load, wear experiments have been conducted for two different engine speeds as 10Hz and 25Hz. In order to observe the wear characteristics and monitor surface conditions of the valve assemblies, a cost-effective 3-D wear analysis system based on the shape from focus(SFF) and machine vision has been fabricated in this paper. 3-D surface topography of the valve assemblies has been analyzed to understand the wear behavior according to operating conditions of the engine. Consequently, wear volume of the valve assemblies is quantized by using the developed 3-D wear analysis system.