The purpose of this study was to identify the change of perception and preference of color according to time passage. The subjects were university students and for the survey 257 questionnaires in 1998 and 522 questionnaires in 2005 were effectively used to compare the perception and preference of color between the both period. The statistics was performed with frequency analysis and independent t-test in SPSS 10.0. The results were as follows. 1) Color perception according to time passage showed significant differences in red, yellow, green, and blue color. 2) Color preference according to time passage showed significant differences in red, green, and blue color. 3) Color perception among three regions according to time passage showed significant differences in red color in Seoul, yellow color in Seoul, Mokpo and Jeju, green color in Seoul and Mokpo, blue color in Seoul, Mokpo and Jeju, and purple color in Seoul. 4) Color preference among three regions according to time passage showed significant differences in yellow color in Mokpo, green and blue color in Seoul, Mokpo and Jeju.