총 아플라톡신 분석을 위한 최적의 추출 및 용출조건을 확립하여 최적의 전처리 조건을 확립하였다. 추출용매는 70% methanol 용액에 NaCl을 각각 1% 첨가한 용액을 사용하였고, 정제용 컬럼인 immunoaffinity column을 이용하여 acetonitrile 3 mL를 용출용매로 사용하여 아플라톡신 혼합용액의 분리능을 좋게 하였다. 검출한계는 0.05 ng/g 수준이였고, 식품의 유형별 총 아플라톡신($B_1,;B_2,;G_1,;G_2$)의 회수율은 곡류 $74.1{sim}95.5%,;83.7{sim}98.8%,;80.4{sim}102.4%$ 및 $72.8{sim}76.5%$, 두류 $85.8~87.5%,;83.8{sim}90.7%,;92.0{sim}94.5%$ 및 $60.6{sim}65.6%$, 견과류 $84.6{sim}97.1%,;86.0{sim}94.1%,;95.5{sim}111.5%$ 및 $71.0{sim}89.9%$, 가공식품 $81.5{sim}87.1%,;82.8{sim}85.8%,;85.4{sim}92.7%$ 및 $68.9{sim}76.4%$, 건조과실류 $83.6{sim}93.5%,;78.1{sim}90.4%,;93.0{sim}108.5%$ 및 $64.9{sim}78.5%$, 기타식품 $72.5{sim}98.3%,;73.1{sim}96.4%,;83.5{sim}107.2%$ 및 $64.2{sim}75.8%$를 보여주었다.
We optimized conditions of extract solvents and elution solvents for total aflatoxins in foods using HPLC/FLD. The extract solvent was 70% methanol solution including 1% NaCl and the 3 mL of acetonitrile was used as elution solvent using immnuoaffinity column. The detection limits (LOD) was 0.05 ng/g. The recoveries for total aflatoxins ($B_1,;B_2,;G_1;and;G_2$) studied in foods were cereals ($74.1{sim}95.5%,;83.7{sim}98.8%,;80.4{sim}102.4%,;72.8{sim}76.5%$), pulses ($85.8{sim}87.5%,;83.8{sim}90.7%,;92.0{sim}94.5%,;60.6{sim}65.6%$), nuts ($84.6{sim}97.1%,;86.0{sim}94.1%,;95.5{sim}111.5%,;71.0{sim}89.9%$), processed foods ($81.5{sim}87.1%,;82.8{sim}85.8%,;85.4{sim}92.7%,;68.9{sim}76.4%$), dried fruits ($83.6{sim}93.5%,;78.1{sim}90.4%,;93.0{sim}108.5%,;64.9{sim}78.5%$) and other foods ($72.5{sim}98.3%,;73.1{sim}96.4%,;83.5{sim}107.2%,;64.2{sim}75.8%$), respectively.
