This study investigated effect of arm posture, wrist motion repetition and external load on perceived discomfort through an experiment. Eleven female college students participated in the experiment, where shoulder, elbow and wrist motion, wrist motion repetition, and external load were used as independent variables. The results showed that only external load had a significant effect on perceived discomfort. The perceived discomfort linearly increased with external load. Based on the results of this and the previous study for males, it was concluded that effect of external load on perceived discomfort was larger than that of other posture and motion repetition related variables. This implies that effect of external load is the most important factor considered in the first place when assessing postural load.