Inspection works are mainly carried out with the help of human sensory organs and are relatively simple and repetitive, so that the workers easily become to feel fatigue and monotony, and their mental activity levels attenuate. Consequently, during the work time, it is natural that various lighting conditions around the workplaces may have in-fluence on work performance. This study aimed to analyze cortical fatigue of inspection workers. Thus, an inspection work was simulated on a computer monitor under various lighting conditions, and CFF, EEG, EOG, and HRV were analyzed. According to the results, fatigue symptoms turned up about $60{sim}90$ minutes after the onset of the work. The work performance also decreased when the fatigue symptoms due to lighting conditions turned up. The variations of fatigue and work performance were affected by illuminators, illumination levels, or interaction of those two factors. The spiral fluorescent lamp seemed improper to the inspection work, because the work performance under that condition was lower than under any other illuminators.