Purpose: The purpose of this study was to explore the effect of Tai Chi exercise on pain, balance, gait and physical function of patients with low back pain. Method: This study was designed one group pretest-posttest design. Tai Chi exercise was conducted by researcher and carried out for sixty minutes per one time and two times a week for six weeks. The subjects of this study consisted of 23 low back patients. Using the SPSS win 10.0 program was used for data analysis, which included frequency, percentage and paired t-test. Result: Tai Chi exercise decreased pain, improved balance and gait, increased physical function(back muscle strength, leg muscle strength and flexibility). Conclusion: The results suggested Tai Chi exercise can be effective nursing intervention to improve pain, balance, gait and physical function of patients with low back pain.