This study was carried out to measure and analyze the characteristics of noise and vibration, and to analyze their effects on the productivity of layers, mechanical troubles, and abnormal wear-out failure of facilities and equipment of the layer house. The measurements of noise and vibration were taken at 13 layer farms nationwide for the operations of feed supplier system, feed distribution system, automatic egg collection system, ventilation system, blot conveyer for layer feces, and fur the case of with and without their operation by a sound level meter and a vibration measuring system in the layer house equipped with upright multi-tier cages. Measurement results showed that normal times were noise(N) 82 dB and vibration(V) 0.2072 cm/s, feed supplier system were 90 dB(N) and 2.8560 cm/s(V), feed distribution system were 90 dB(N) and 2.0222 cm/s(V), automatic egg collection system were 87 dB(N) and 0.1865 cm/s(V), ventilation system 88 dB(N) and 2.5364 cm/s(V), belt conveyer fur layer feces were 88 dB(N) and 0.2387 cm/s(V), and then maximum values of noise and vibration were 90 dB and 2.8560 cm/s, respectively, when feeding systems(feed supplying system and feed distribution system) were operated. Based on these results, an experiment is being conducted to find out the effect of noise and vibration on the productivity of layers in the layer house equipped with upright multi-tier cages.