In this study, the effect of fixation methods, relaxation treatment and washing treatment on the comfortable properties of MDPPA/HMM treated cotton weft-knitted fabrics was studied. As a result, 1) Air permeability of knitted fabrics decreased after flame resistant treatment. Single jersey and 10G showed higher value of air permeability than those of interlock and 14G. Swelling agent and wet fixation method which increased the values of W/T and decreased the values of WC/W resulted in decreased values of air permeability. 2) $Q_{max}$ of knitted fabrics decreased after washing treatment. $Q_{max}$ of knitted fabrics increased with decreasing air permeability 14G showed higher value of $Q_{max}$ than those of 10G. 3) Water absorption of knitted fabrics increased after relaxation treatment. Water absorption of knitted fabrics increased with increasing the value of W/T. Water absorption of knitted fabrics is more affected by the structure of fabrics rather than the effect of treatment.