In this paper, anode supported SOFC with columnar structured YSZ electrolyte was fabricated via Electron Beam Physical Vapor Deposition (EBPVD) method. Liquid condensation process was employed for the preparation of NiO-YSZ substrate and the high power electron beam deposition method was used for the deposition of YSZ electrolyte film. Double layered cathode with LSM-YSZ and LSM was printed on electrolyte via screen-printing method and fired at $1150^{circ}C$ in air atmosphere for 3 h. The electrochemical performance and the long-term stability of $5{ imes}5cm^2$ single cell were investigated with DC current-voltage characteristics and AC-impedance spectroscopy. According to the investigation, $5{ imes}5cm^2$ sized unit cell showed the maximum power density of around $0.76W/cm^2$ at $800^{circ}C$ and maintained the stable performance over 400 h.