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Identification of Ectomycorrhizal Fungi from Pinus densiflora Seedlings at an Abandoned Coal Mining Spoils
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  • Identification of Ectomycorrhizal Fungi from Pinus densiflora Seedlings at an Abandoned Coal Mining Spoils
  • Identification of Ectomycorrhizal Fungi from Pinus densiflora Seedlings at an Abandoned Coal Mining Spoils
저자명
Park. Sang-Hyeon,Jeong. Hyeon-Suk,Lee. Yoo-Mee,Eom. Ahn-Heum,Lee. Chang-Seok
간행물명
Journal of ecology and field biology : JEFB
권/호정보
2006년|29권 2호|pp.143-149 (7 pages)
발행정보
한국생태학회
파일정보
정기간행물|ENG|
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기타
이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
서지반출

기타언어초록

This study was conducted to identify native ectomycorrhizal (ECM) fungi colonizing Pinus densiflora for revegetation of abandoned coal mines in Korea. Seedlings of P. densiflora growing on coal mining spoils of a study site in Samcheok were collected. ECM roots were observed under stereomicroscope and their DNA were extracted from each root tip for a seedling for molecular identification. A PCR primer pair specific to fungi, ITS1F and ITS4, was used to amplify fungal DNA. Restriction enzymes, Alul and Hinfl were used for restriction fragment length polymorphism (RFLP). Combined with RFLP profiles and sequence analysis, total twenty one taxa were identified from the ECM root tips. Basidiomycetous fungi including Thelephoraceae, Pezizales, Laccaria, Pisolithus and Ascomycetous fungi including ericoid mycorrhizal fungi were identified from this study. Results showed that the most frequently found in the study sites was a species in Thelephoraceae. A possible use of ECM fungi identified in this study for the revegetation of abandoned coal mines with P. densiflora was discussed.