The purpose of this study is to analyze the subjective sensations and preference for spring/fall casual cotton shirt fabrics, and to assist in developing shirt fabrics that offer maximum comfort in wearing sensation. The subjects of this study were male and female college students who assessed the tactile sensation of seven types of spring/fall shirt fabrics available on the market: plain weave and rib weave made of cotton and that of cotton mixed fabrics. The subjects were university students between the ages of 20 to 23, who were residing in the city of Cheongju, Korea. The questionnaires related to the fabrics hand were evaluated using a nine point bi-polar scale of 9 descriptive terms. Mean, t-test, F-test and $X^2$ were used for data analysis. The fabric hand evaluation on the seven types of shirts showed a significant difference in all of the 9 descriptive terms. There were partially significant differences in the subjective sensations according to fabric characteristics regarding shirt fabrics. As regards the difference in fabric hand according to gender, female students responded with more receptiveness and sensitivity than male students in the category of woven fabric evaluation. In particular, female students responded with more sensitivity to the sensation of dense and wrinkle sensations. There were significant differences in the purchase preference according to gender regarding casual shirt fabrics. Plain weave fabrics made of cotton was most preferred among male students, whereas rib weave fabrics made of cotton/polyester was most preferred among female students.