The purpose of this study was to ascertain the prey composition and utilization of the Eurasian otter, Lutra lutra, in the ecosystem conservation region of the Seomjin River. The study was conducted for a 12-month period from March 2003 to February 2004 by spraints analysis and an investigation using artificial holts. We found seasonal variation in the number of spraints and composition of prey items. Fish were the main prey items throughout the period (82%), and other prey items included birds (14%), amphibians (2%) and arthropods (1%). In addition to prey identification, we investigated habitat utilization by otters through spraints distribution. The traces of otters were discovered in five of the six total artificial holts. However, the utilization of the artificial holts in the study area seemed to be limited as evidenced by the low frequency of traces in the holts.