In this paper, we address an extended hub-and-spoke transportation network design problem (EHSNP). In the existing hub location problems, the location and number of spokes, and shipments on spokes are given as input data. These may, however, be viewed as the variables according to the areas which they cover. Also, the vehicle routing in each spoke needs to be considered to estimate the network cost more correctly. The EHSNP is a problem of finding the location of hubs and spokes, and pickup/delivery routes from each spoke, while minimizing the total related transportation cost in the network. The EHSNP is an integrated problem that consists of several interrelated sub-problems. To solve EHSNP, we present an approach based on a symbiotic evolutionary algorithm (symbiotic EA), which are known as an efficient tool to solve complex integrated optimization problems. First, we propose a framework of symbiotic EA for EHSNP and its genetic elements suitable for each sub-problem. To analyze the proposed algorithm, the extensive experiments are performed with various test-bed problems. The results show that the proposed algorithm is promising in solving the EHSNP.