The present Study investigated on effect of working time and physical work environment on job satisfaction, and is based on a representative survey carried out in the 15 European Union Member States in 2,000 on behalf of the European Foundation for the Improvement of Living and Working Conditions by Infratest Burke Sozialforschung and a consortium of national fieldwork institutes. In the course of the survey, a total of 21,703 people drawn from the economically active populations in the 15 countries were questioned about their working time, overtime, weekend work, physical work environment, social support of supervisor and co-worker and job satisfaction. Regression analysis and subgroup analysis were employed to examine the hypotheses. It was found that weekend work, overtime and physical work environment associate negatively with job satisfaction. Social support of supervisor and co-worker moderated only relation between working time and job satisfaction. The results provide support for prior quantitative studies partly. Based on these significant research findings, theoretical implications were discussed.