The main purpose of this study is to identify the factors affecting personnel e-Learning acceptance. We used self-efficacy and perceived playfulness as independent variables to extended Technology Acceptance Model in an e-Learning contort. Perceived playfulness is the one of the important personal intrinsic motivational factors which could enlarge the understanding process of technology acceptance in the personal perspective. Questionnaires were collected from 94 respondents who experienced e-Learning systems that L Corporation provided. This study developed and empirically tested a model representing the relationship by using Structural Equation Model among factors which are: perceived usefulness, perceived ease of use, perceived playfulness, self-efficacy and attitudes. Also we measured affective and cognitive attitude because, most psychometric literature explain attitude has two different dimensions which are affective and cognitive. The major findings of the study are summarized as follows. Firstly, the higher the perceived usefulness the more affected the behavioral intention was to use as well as attitude to be positive. Secondly, as much as perceived playfulness more affected the perceived ease of use as well as self-efficacy. Finally, the perceived ease of use and self-efficacy are not significantly affected attitude while they are affected perceived usefulness and perceived playfulness.