A probable case of superfetation in a Korean native cow met in a small farm located in Imsil Gun, Chonbuk. The cow delivered twice a living male and female calves in September 4 and December 9, 2004, respectively. Thus, we determined whether this case is a case of superfetation using parentage testing technique. The parentage testing was carried out for a dam and two calves using microsatellite DNA and blood typing. As the calves had at least one of the alleles on all marker tested that existed in dam, it was estimated that both of the calves were offsprings of the cow, and that they came from superfetation.